B-Cell Linker anticorps (pTyr84) (Alexa Fluor 488)

N° du produit ABIN1177038

Cet anticorps anti-B-Cell Linker est un anticorps Souris Monoclonal détectant B-Cell Linker dans ICS. Adapté pour Humain et Souris. Ce Primary Antibody a été cité dans 1 publication.

Aperçu rapide pour B-Cell Linker anticorps (pTyr84) (Alexa Fluor 488) (ABIN1177038)

Antigène: B-Cell Linker (BLNK)

Reactivité: Humain, Souris

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp B-Cell Linker est conjugé à/à la Alexa Fluor 488

Application: Intracellular Staining (ICS)

Clone: J117-1278

Détail du produit anti-B-Cell Linker anticorps (Alexa Fluor 488)

Épitope: pTyr84

Marque: BD Phosflow™

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogène: Phosphorylated Human BLNK Peptide

Isotype: IgG2b kappa

Information d'application

Volume d'échantillon: 20 μL

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: Aqueous buffered solution containing BSA and ≤0.09 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Stockage commentaire: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The antibody was conjugated to Alexa Fluor® 488 under optimum conditions, and unreacted Alexa Fluor® 488 was removed.

Références pour anticorps anti-B-Cell Linker (Alexa Fluor 488) (ABIN1177038)

Chiu, Dalton, Ishiai, Kurosaki, Chan: "BLNK: molecular scaffolding through 'cis'-mediated organization of signaling proteins." dans: The EMBO journal, Vol. 21, Issue 23, pp. 6461-72, (2002) (PubMed).

Détails sur B-Cell Linker

Antigène: B-Cell Linker (BLNK)

Autre désignation: BLNK

Sujet: B cell activation is initiated by crosslinking the B cell receptor, which leads to activation of non-receptor protein tyrosine kinases (PTK), including Btk, Syk, and three Src kinases, Fyn, Lyn, and Blk. Activated PTKs then phosphorylate multiple cellular proteins involved in B lymphocyte signaling. Syk is responsible for the tyrosine phosphorylation of B cell linker protein (BLNK), a member of the SLP-76 family of adapter proteins. Phosphorylation of human BLNK at tyrosines 84, 178, and 189 (Y84, Y178, and Y189) creates docking sites for PLCg2, leading to the activation of downstream signaling pathways. The J117-1278 monoclonal antibody recognizes the phosphorylated Y84 of BLNK.

Pathways: BCR Signaling